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It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.
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The capacity of each node (how many points it can hold before splitting) controls the shape of the tree. A low capacity means nodes split early, producing a deep tree with many small cells. A high capacity means nodes tolerate more points before splitting, producing a shallow tree with larger cells.