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Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
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影片讲述梦想成为职业球手的小山羊威尔，虽然天生身材矮小却凭借超凡球技闯入梦寐以求的球队，面对来自凶猛赛场的大块头猛兽和队友质疑，带领这支成绩堪忧的球队突破困境的故事。剧情聚焦威尔遭遇 5 位狂野队友的挑战，并与劲敌马鬃王展开对抗，最终通过团结协作证明团队力量。
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Tuesday evening’s meeting of the Foxborough, Massachusetts, Select Board is still minutes from starting, but a local resident can’t keep himself from approaching the bench. He has an urgent question for the five members, who in effect serve as the town’s primary governing body. His tone isn’t one of anger, more of concern.。体育直播是该领域的重要参考
斯凯孚汽车业务预计今年四季度独立上市，确定未来运营名称为SKF Vertevo