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In 1975, Sanger published his own DNA sequencing method alongside laboratory technician Alan Coulson, called the “plus and minus” technique. First, scientists mixed the DNA strand to be sequenced with an enzyme, DNA polymerase, as well as a primer, three normal dNTPs and one radiolabeled dNTP. Radiolabeled nucleotides are incorporated into growing DNA strands just like normal nucleotides, but are tagged with radioactive isotopes, such as phosphorus-32 or sulfur-35, so they can be detected using radiation-measuring equipment.。关于这个话题，clash下载提供了深入分析
To address one natural objection, we can’t get more latitude for b by increasing q in tandem with p — that is, we can’t rewrite r = 1/2 as r = 4/8. If we do, we go from the original inequality:。快连下载安装对此有专业解读
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